论文标题
$ \ textit {escherichia coli} $的法规基因组一次，一次是一百个发起人
Deciphering the regulatory genome of $\textit{Escherichia coli}$, one hundred promoters at a time
论文作者
论文摘要
DNA测序的进步彻底改变了我们阅读基因组的能力。但是，即使在最深思的生物体中，细菌$ {\ it escherichia coli} $，对于$ \ $ \ $ 65 $ \％$ \％的发起人，我们仍然完全不了解其法规。在我们破解了这种调节性的罗塞塔石头之前，读写基因组的努力将保持偶然。我们介绍了一种新方法（Reg-Seq），该方法将大量并行的报告基因测定法和质谱分析链接，以在12种不同的生长条件下以$ {\ it E. coli} $中的100多个启动子的基础分辨率解析。首先，我们表明我们的方法从已知序列中概括了调节信息。然后，我们检查了$ {\ it e.coli} $基因组中80多个启动子的调节架构，该基因组以前没有已知的法规。在许多情况下，我们还确定哪些转录因子介导了其调节。此处介绍的方法清除了一条充分表征模型生物的调节基因组的途径，并有可能进入其他生态和医学相关性的其他微生物。
Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium ${\it Escherichia coli}$, for $\approx$ 65$\%$ of the promoters we remain completely ignorant of their regulation. Until we have cracked this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method (Reg-Seq) linking a massively-parallel reporter assay and mass spectrometry to produce a base pair resolution dissection of more than 100 promoters in ${\it E. coli}$ in 12 different growth conditions. First, we show that our method recapitulates regulatory information from known sequences. Then, we examine the regulatory architectures for more than 80 promoters in the ${\it E. coli}$ genome which previously had no known regulation. In many cases, we also identify which transcription factors mediate their regulation. The method introduced here clears a path for fully characterizing the regulatory genome of model organisms, with the potential of moving on to an array of other microbes of ecological and medical relevance.
