论文标题
深入了解二核体的拆开
Insight into the Unwrapping of the Dinucleosome
论文作者
论文摘要
核小体的动力学是染色质的组成部分，对真核生物中基因组的表达，复制和修复具有至关重要的影响。除了通过热波动的核小体恒定运动外，ATP依赖性染色质重塑络合物会导致其活跃位移。在这里，我们提出了在存在外力的情况下对二核体包装和解开动态的理论分析。我们探索不同未包装状态下的二核体的能量景观和配置。此外，使用动态的蒙特卡洛模拟算法，我们演示了系统的动力学特征，例如用于部分和完整包装过程的解开力。此外，我们表明，在较短的链接器DNA（$ \ sim 10-90 $ bp）中，不对称拆卸会发生。这些发现可能会阐明染色质动力学和基因可及性。
Dynamics of nucleosomes, the building blocks of the chromatin, has crucial effects on expression, replication and repair of genomes in eukaryotes. Beside constant movements of nucleosomes by thermal fluctuations, ATP-dependent chromatin remodelling complexes cause their active displacements. Here we propose a theoretical analysis of dinucleosome wrapping and unwrapping dynamics in the presence of an external force. We explore the energy landscape and configurations of dinucleosome in different unwrapped states. Moreover, using a dynamical Monte-Carlo simulation algorithm, we demonstrate the dynamical features of the system such as the unwrapping force for partial and full wrapping processes. Furthermore, we show that in the short length of linker DNA ($\sim 10 - 90$ bp), the asymmetric unwrapping occurs. These findings could shed some light on chromatin dynamics and gene accessibility.
