论文标题
肌动蛋白网络形成的动力学通过时间分辨的粒子跟踪微流变学测量
Kinetics of actin networks formation measured by time resolved particle-tracking microrheology
论文作者
论文摘要
肌动蛋白是研究最多的细胞骨架蛋白之一，显示出非常丰富的结构。它可以自我组装成主导细胞机械性能的动力结构，其运动性和分裂。但是，只有很少的研究表征了活跃肌动蛋白自组装过程的动力学，而不是纠缠网络的形成。在这里，我们遵循肌动蛋白聚合动力学和组织，使用时间解决的被动微流变学进入纠缠网络。我们建立了单体的初始浓度，主动聚合和网络形成动力学与粘弹性特性从肌动蛋白聚合开始到稳态纠缠网络的形成。令人惊讶的是，我们发现，在足够高的初始单体浓度下，形成肌动蛋白网络的弹性模量超出了大声，然后使用-2/5功率定律放松，我们将网络的重排归因于稳态结构。
Actin is one of the most studied cytoskeleton proteins showing a very rich span of structures. It can self-assemble actively into dynamical structures that govern the mechanical properties of the cell, its motility and its division. However, only very few studies characterize the kinetics of the active actin self-assembly process beyond the formation of an entangled network. Here, we follow actin polymerization kinetics and organization into entangled networks using time resolved passive microrheology. We establish a relationship between the initial concentration of monomers, the active polymerization and network formation kinetics, and the viscoelastic properties from the onset of actin polymerization upto the formation of a steady state entangled network. Surprisingly, we find that at high enough initial monomer concentrations the elastic modulus of the forming actin networks overshoots and then relaxes with a -2/5 power law, that we attribute to rearrangements of the network into a steady state structure.
