论文标题
通过石墨烯场效应晶体管的COVID-19峰蛋白受体结合结构域的尖峰蛋白受体结合结构域的电探测
Electrical probing of COVID-19 spike protein receptor binding domain via a graphene field-effect transistor
论文作者
论文摘要
在这里，为了对2019年新型冠状病毒病（COVID-19）进行便捷和快速筛查/诊断，我们将非精神敏感的石墨烯场晶体晶体管（GR-FET）与高度选择性的抗体 - 抗原抗原相互作用相结合，以开发冠状病毒免疫传感器。 GR-FET免疫传感器可以快速识别（约2分钟），并以实时和无标记的方式准确捕获Covid-19-Spike蛋白S1（包含受体结合域，RBD），以降低至0.2 pm。进一步的结果确保了GR-FET免疫传感器可以在浓度下降到0.1 pm的高亲和力抗体（结合常数高达2*10^11 m^-1）的筛选。因此，我们开发的电气GR-FET免疫传感器提供了一种吸引人的替代方法，可解决早期筛查/诊断，以及这种正在进行的公共卫生危机的中和抗体锁定方法的分析和合理设计。
Here, in an effort towards facile and fast screening/diagnosis of novel coronavirus disease 2019 (COVID-19), we combined the unprecedently sensitive graphene field-effect transistor (Gr-FET) with highly selective antibody-antigen interaction to develop a coronavirus immunosensor. The Gr-FET immunosensors can rapidly identify (about 2 mins) and accurately capture the COVID-19 spike protein S1 (which contains a receptor binding domain, RBD) at a limit of detection down to 0.2 pM, in a real-time and label-free manner. Further results ensure that the Gr-FET immunosensors can be promisingly applied to screen for high-affinity antibodies (with binding constant up to 2*10^11 M^-1 against the RBD) at concentrations down to 0.1 pM. Thus, our developed electrical Gr-FET immunosensors provide an appealing alternative to address the early screening/diagnosis as well as the analysis and rational design of neutralizing-antibody locking methods of this ongoing public health crisis.
