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We present multi-wavelength measurements of the thermal, chemical, and cloud contrasts associated with the visibly dark formations (also known as 5-$μ$m hot spots) and intervening bright plumes on the boundary between Jupiter's Equatorial Zone (EZ) and North Equatorial Belt (NEB). Observations made by the TEXES 5-20 $μ$m spectrometer at the Gemini North Telescope in March 2017 reveal the upper-tropospheric properties of 12 hot spots, which are directly compared to measurements by Juno using the Microwave Radiometer (MWR), JIRAM at 5 $μ$m, and JunoCam visible images. MWR and thermal-infrared spectroscopic results are consistent near 0.7 bar. Mid-infrared-derived aerosol opacity is consistent with that inferred from visible-albedo and 5-$μ$m opacity maps. Aerosol contrasts, the defining characteristics of the cloudy plumes and aerosol-depleted hot spots, are not a good proxy for microwave brightness. The hot spots are neither uniformly warmer nor ammonia-depleted compared to their surroundings at $p<1$ bar. At 0.7 bar, the microwave brightness at the edges of hot spots is comparable to other features within the NEB. Conversely, hot spots are brighter at 1.5 bar, signifying either warm temperatures and/or depleted NH$_3$ at depth. Temperatures and ammonia are spatially variable within the hot spots, so the precise location of the observations matters to their interpretation. Reflective plumes sometimes have enhanced NH$_3$, cold temperatures, and elevated aerosol opacity, but each plume appears different. Neither plumes nor hot spots had microwave signatures in channels sensing $p>10$ bars, suggesting that the hot-spot/plume wave is a relatively shallow feature.