论文标题
饲养密度对Gilthead Sea Bream（Sparus Aurata，L。1758）的生长，消化条件，福利指标和肠道细菌群落的影响
Effects of rearing density on growth, digestive conditions, welfare indicators and gut bacterial community of gilthead sea bream (Sparus aurata, L. 1758) fed different fishmeal and fish oil dietary levels
论文作者
论文摘要
在地中海水产养殖中，很少的研究研究了饲养密度与饮食成分之间的相互作用，对主要的关键性能指标，生理过程和肠道细菌群落之间的相互作用。因此，进行了一项研究，以评估生长反应，消化酶活性，对粘液的体液免疫力，血浆生物化学和Gilthead Sea Bream（Sparus aurata，L. 1758）的肠道菌群，在高（HD）高（HD）和低（LD）最终储存量和最终库存量（LD）最终库存量（FM）30％（FM 30％）中，FLM 30％，FLM 30％，FM 30％，FM30％，FM30％，FM30％，FM 30％，FM 30％FMEAR，FIOL FRM 15％。 FO）和低（FM10FO3； 10％FM和3％FO）FM和FO水平。喂食异义源和分离的挤出饮食，以三式三份的鱼类（初始重量：96.2 g）在98天内过度喂食。所测试的密度对在高或低FM和FO饮食水平饲养的海洋的总体生长和饲料效率没有重大影响。消化酶活性的结果表明，饲养密度和每种饮食治疗中的消化效率可比。与营养和生理状况有关的血浆参数在两种营养条件下的饲养密度不受影响，也通过研究溶菌酶，蛋白酶，抗脂蛋白，抗脂蛋白和总蛋白质确定皮肤粘液中的首次对这种物种上的溶菌酶，蛋白酶，抗脂肪酶和总蛋白质确定来实现类似的观察，这是对这种物种的首次在这种物种上，对饲养密度群体的效果进行了研究。检测到与HD和LD下的饮食治疗有关的不同反应。低FM-FO饮食保持稳定的LD和HD状况之间的肠道细菌群落的生物多样性，而FIS喂养高FM-FO水平的鱼类表现出HD时生物多样性的降低。根据结果，以低或高的FM-FO饮食的密度为36-44 kg M3的密度，在生长阶段的吉尔特头海oe骨似乎是可行的，而不会对生长，饲料效率，福利状况和肠道细菌群落产生负面影响。
In Mediterranean aquaculture little research has examined the interaction between rearing density and dietary composition on main key performance indicators, physiological processes and gut bacterial community. A study was undertaken, therefore to assess growth response, digestive enzyme activity, humoral immunity on skin mucus, plasma biochemistry and gut microbiota of gilthead sea bream (Sparus aurata, L. 1758) reared at high (HD) and low (LD) final stocking densities and fed high (FM30FO15, 30 % fishmeal FM, 15 % fish oil, FO) and low (FM10FO3; 10 % FM and 3 % FO) FM and FO levels. Isonitrogenous and isolipidic extruded diets were fed to triplicate fish groups (initial weight: 96.2 g) to overfeeding over 98 days. The densities tested had no major effects on overall growth and feed efficiency of sea bream reared at high or low FM and FO dietary level. Results of digestive enzyme activity indicated a comparable digestive efficiency among rearing densities and within each dietary treatment. Plasma parameters related to nutritional and physiological conditions were not affected by rearing densities under both nutritional conditions a similar observation was also achieved through the study of lysozyme, protease, antiprotease and total protein determination in skin mucus, For the first time on this species, the effect of rearing density on gut bacterial community was studied. Different response in relation to dietary treatment under HD and LD were detected. Low FM-FO diet maintained steady the biodiversity of the gut bacterial community between LD and HD conditions while fish fed high FM-FO level showed a reduced biodiversity at HD. According to the results, it seems feasible to rear gilthead sea bream at the on-growing phase at a density up to 36-44 kg m3 with low or high FM-FO diet without negatively affecting growth, feed efficiency, welfare condition and gut bacterial community.
