论文标题
两个时间尺度控制细胞中大蛋白质聚集体的产生
Two timescales control the creation of large protein aggregates in cells
论文作者
论文摘要
蛋白质聚集特别令人感兴趣，因为它与许多疾病和疾病的联系。许多因素可以改变此过程的动态和结果，其中之一是系统中单体和聚集体的扩散率。在这里，我们从实验和理论上研究细胞中的聚集过程，并确定了两个不同的物理时间尺度，这些时间表设置了聚集体的数量和大小。第一个时间尺度涉及小簇在细胞质中自由扩散的快速聚集，而在第二个簇中，聚集体大于细胞质的孔径，因此几乎没有扩散，并且聚集过程减慢了。但是，该过程并非完全停止，可能反映出无数的活跃但随机力引起了聚集体。这种缓慢的时间尺度对于考虑聚合过程的实验结果至关重要。这些结果也可能对细胞生物学的其他空间组织的其他过程（例如相分离的液滴）产生影响。
Protein aggregation is of particular interest due to its connection with many diseases and disorders. Many factors can alter the dynamics and result of this process, one of them being the diffusivity of the monomers and aggregates in the system. Here, we study experimentally and theoretically an aggregation process in cells, and we identify two distinct physical timescales that set the number and size of aggregates. The first timescale involves fast aggregation of small clusters freely diffusing in the cytoplasm, while, in the second one, the aggregates are larger than the pore size of the cytoplasm and thus barely diffuse, and the aggregation process is slowed down. However, the process is not entirely halted, potentially reflecting a myriad of active but random forces forces that stir the aggregates. Such slow timescale is essential to account for the experimental results of the aggregation process. These results could also have implications in other processes of spatial organization in cell biology, such as phase-separated droplets.
