论文标题
用于中尺度神经元网络体外工程的PDMS微流体膜
PDMS microfluidic film for in vitro engineering of mesoscale neuronal networks
论文作者
论文摘要
聚二甲基硅氧烷（PDMS）微流体设备已成为工程细胞和多细胞网络的标准工具。但是，通常使用活检打孔器手动制造储层或细胞访问设备的整个孔，因此很难创建一个大型的小（<1 mm）储层。在这里，我们提出了用于薄膜微流体设备或微流体膜（μFF）的制造工艺，其中包含一系列整个孔。表征了10 mm乘10 mm的小至100μmx100μm的孔。整个孔的几何形状由光震杆精确定义。使用μFF进行细胞培养的挑战是整个孔中的气泡捕集，这在较小的孔中变得更加突出。我们表明，可以使用乙醇介导的PDM表面润湿来克服这个问题，并证明在μFFs中生长的培养神经元网络的功能记录。这项技术为微流体设备的新应用打开了由数十至数百个单元组成的中尺度系统。
Polydimethylsiloxane (PDMS) microfluidic devices have become a standard tool for engineering cells and multicellular networks in vitro. However, the reservoirs, or through-holes where cells access the devices, are usually fabricated manually using a biopsy punch, making it difficult to create a large-scale array of small (<1 mm) reservoirs. Here, we present a fabrication process for a thin-film microfluidic device, or a microfluidic film (μFF), containing an array of through-holes. Holes as small as 100 μm by 100 μm spanning 10 mm by 10 mm are characterized. The geometry of the through-holes was precisely defined by the photoresist mould. A challenge in using the μFF for cell culture was air-bubble entrapments in the through-holes, which became more prominent with smaller holes. We show that this issue can be overcome using ethanol-mediated wetting of the PDMS surface, and demonstrate functional recording of cultured neuronal networks grown in μFFs. This technology opens new application of microfluidic devices to mesoscale systems comprised of several tens to hundreds of cells.
