论文标题
多细胞球体在渗透压缩下的机械行为
Mechanical behavior of multi-cellular spheroids under osmotic compression
论文作者
论文摘要
内部和外部机械环境在肿瘤发生中起重要作用。作为血管早期状态肿瘤的代理，我们使用多细胞球体，由细胞制成的复合材料，细胞外基质和渗透液。我们通过用渗透冲击压缩聚集体并使用活性的毛弹性材料来对骨料进行压缩，从而在数分钟至小时的时间内表征其有效的流变学，从而重现聚集体中的应力和应变分布。该模型还预测了聚集体的新出现大量模量以及渗透性间质流体的液压扩散是如何通过聚集体内的活跃应力来改变的。我们进一步表明，这两个现象学参数的价值可以通过考虑到在我们的实验环境中有效不可渗透和不可压缩的包含物来合理化，并嵌套在可压缩且可渗透的矩阵中。
The internal and external mechanical environment plays an important role in tumorogenesis. As a proxy of an avascular early state tumor, we use multicellular spheroids, a composite material made of cells, extracellular matrix and permeating fluid. We characterize its effective rheology at the timescale of minutes to hours by compressing the aggregates with osmotic shocks and modeling the experimental results with an active poroelastic material that reproduces the stress and strain distributions in the aggregate. The model also predicts how the emergent bulk modulus of the aggregate as well as the hydraulic diffusion of the percolating interstitial fluid are modified by the preexisting active stress within the aggregate. We further show that the value of these two phenomenological parameters can be rationalized by considering that, in our experimental context, the cells are effectively impermeable and incompressible inclusions nested in a compressible and permeable matrix.
