论文标题
高功率接近浓度的Fabry-Perot腔，用于相对造影剂显微镜
High-Power Near-Concentric Fabry-Perot Cavity for Phase Contrast Electron Microscopy
论文作者
论文摘要
玻璃化生物大分子（Cryo-EM）的透射电子显微镜（TEM）受这些样品可用的弱相对比信号的限制。因此，使用相板将基本上改善信噪比。我们以前已经证明了将大功率Fabry-Perrot腔用作TEM的相板。现在，我们报告对激光腔的改进，使我们能够实现超过450 gw/cm $^{2} $的记录连续波强度，足以生成300 keV电子的最佳90°相移。此外，我们使用激光相板进行了第一个冷冻EM重建，这表明该激光相板的稳定性足以在标准的低温EM数据收集中使用。
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous-wave intensities of over 450 GW/cm$^{2}$, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.
