论文标题
反射模式光学衍射断层扫描，用于厚生物标本的无标签成像
Reflection-mode optical diffraction tomography for label-free imaging of thick biological specimens
论文作者
论文摘要
光学衍射断层扫描（ODT）已成为一种强大的无标签的三维（3D）生物成像技术，用于观察活细胞和薄组织层。我们报告了一种新的反射模式ODT（RODT）方法，用于成像具有500 nm横向分辨率和1μM轴向分辨率的厚的生物样品。在RODT中，通过动态斑点场干涉法提供的时空门控拒绝了多个散射背景，而深度分辨的折射率图是通过开发全面的反向散射模型来重建的，该模型还考虑了样品诱导的差异。受益于RODT的高分辨率和全场定量成像能力，我们成功地成像了红细胞，并量化了它们的膜波动，并在浑浊样品后面，其厚度为2.8散射平均无路径。我们进一步实现了在离体大鼠眼内角膜的体积成像，并量化了其光学特性，包括在纳米尺度上绘制DUA的地形和Descemet的膜表面。
Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick biological specimens with 500 nm lateral resolution and 1 μm axial resolution. In rODT, multiple scattering background is rejected through spatio-temporal gating provided by dynamic speckle-field interferometry, while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse scattering model that also considers specimen-induced aberration. Benefiting from the high-resolution and full-field quantitative imaging capabilities of rODT, we succeeded in imaging red blood cells and quantifying their membrane fluctuations behind a turbid sample with a thickness of 2.8 scattering mean-free-paths. We further realized volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties, including mapping the topography of Dua's and Descemet's membrane surfaces on the nanometer scale.
