论文标题
等离子碗形纳米孔中单个生物分子的纳米流体捕获和增强的拉曼检测
Nanofluidic trapping and enhanced Raman detection of single biomolecules in plasmonic bowl-shaped nanopore
论文作者
论文摘要
固态纳米孔是单分子蛋白测序的新兴平台，因为它们对哈希生理环境的耐受性以及与不同的电气和光学检测方法的兼容性。但是，它们遭受了不良的分子操作，这些操作与检测方法扭曲并因此受到限制。在这里，我们报告了硝化硅硅上的碗形等离子金纳米孔，以证明对等离子体增强的拉曼光谱检测的DNA易位的近场纳米流体操纵。水凝胶线性化了DNA，并将线性DNA捕获在纳米孔中，以数十秒钟的速度，因此纳米孔的双极效应产生了电渗鞘流动和双极表面电荷分布。它们的组合导致了纳米孔热点中DNA的近场限制，以允许稳定的拉曼检测。我们设想，拉曼光谱与碗形纳米孔的结合可以以无标签的方式在单分子蛋白测序中成功
Solid-state nanopores are emerging platforms for single-molecule protein sequencing due to their tolerance to hash physiology environment and compatibility with different electrical and optical detection methods. However, they suffer from poor molecular manipulations that were twisted with and thus limited by the detection methods. Here, we report a bowl-shaped plasmonic gold nanopore on silicon nitride with hydrogel to demonstrate near-field nanofluidic manipulation of DNA translocation for plasmon-enhanced Raman spectroscopic detection. The hydrogel linearized the DNA, and the linear DNA was trapped in the nanopore for tens of seconds due assumably to bipolar effect of the nanopore that generate electroosmotic sheath flow and bipolar surface charge distribution. Their combination led to a near-field confinement of the DNA in the nanopore hot spot to allow stable Raman detection. We envision that a combination of Raman spectroscopy with the bowl-shaped nanopores can succeed in single-molecule protein sequencing in a label-free way
