论文标题
连续波多通型成像流式细胞术
Continuous wave multi-pass imaging flow cytometry
论文作者
论文摘要
我们提出了无标签成像流式细胞术的宽场多通实现。我们的技术显示了最多四个通过的人类红细胞集合的高速流量成像，显示了对比度和信噪比的X4增强。我们表明，我们的技术接近测量灵敏度的量子极限，将最佳成像的范围扩展到了弱吸收方案中的样品。这允许在有限的照明强度下成像动态样本的实际情况下，允许几乎最佳的成像敏感性和吞吐量，超过了当前可用的量子光源实现的灵敏度。
We present a wide-field multi-pass implementation of label-free imaging flow cytometry. Our technique is shown for high-speed flow imaging of ensembles of human red blood cells with up to four passes, demonstrating x4 enhancement in contrast and signal-to-noise. We show that our technique approaches close to the quantum limit of measurement sensitivity, extending the range of optimal imaging to samples in the weakly absorbing regime. This allows for near optimal imaging sensitivity and throughput in a practical scenario of imaging a dynamic sample under limited illumination intensity, surpassing the sensitivity achieved with currently available quantum light sources.
