ICS 65.020 CCS B 16 湖 DB43 南 省 地 方 标 准 DB43/T 1 9 68—2020 穗状狐尾藻生长抑制试验操作规程 Guideline for Myriophyllum Verticillatum L. Growth Inhibition Test 2020-12-29发布 湖南省市场监督管理局 2021-03-29实施 发 布 DB43/T 1968—2020 目 次 前言························································································································ Ⅲ 1 范围 ····················································································································· 1 2 规范性引用文件 ······································································································ 1 3 术语和定义 ············································································································ 1 3.1 扦插 ··············································································································· 1 3.2 预培养 ············································································································ 1 3.3 植物长 ············································································································ 1 3.4 植物鲜重 ········································································································· 1 3.5 植物干重 ········································································································· 1 4 试验条件 ··············································································································· 2 4.1 温度 ··············································································································· 2 4.2 光照 ··············································································································· 2 4.3 曝气 ··············································································································· 2 4.4 暴露方式 ········································································································· 2 4.5 试验周期 ········································································································· 2 5 材料准备 ·············································································································· 2 5.1 沉积物的制备 ··································································································· 2 5.2 培养基的制备 ··································································································· 2 5.3 生物试材的预培养 ····························································································· 2 6 试验仪器设备 ········································································································· 3 7 试验方法 ··············································································································· 3 7.1 试验准备 ········································································································· 3 7.2 预试验 ············································································································ 3 7.3 正试验 ············································································································ 3 7.4 限度试验 ········································································································· 3 7.5 参比试验 ········································································································· 3 8 观察记录 ··············································································································· 3 9 浓度检测 ··············································································································· 4 10 数据处理·············································································································· 4 11 质量保证与质量控制 ······························································································ 5 附录 A（资料性） SMART and BARKO 培养基配制方法 ························································· 6 附录 B（资料性） 预培养土培法“沉积物-狐尾藻”系统图 ················································ 7 附录 C（资料性） 试验“沉积物-狐尾藻”系统图····························································· 8 I DB43/T 1968—2020 II DB43/T 1968—2020 前 言 本文件按照 GB/T 1.1—2020 给出的规则起草。 本文件某些内容可能涉及专利。本文件发布机构不承担识别这些专利的责任。 本文件由湖南省农业农村厅提出。 本文件由湖南省农业标准化技术委员会归口。 本文件起草单位：湖南省植物保护研究所,长沙艾格里生物科技有限公司。 本文件主要起草人：刘勇、陈昂、张德咏、张战泓、蒋桂芳、罗杰、熊浩、陈武瑛、罗香文。 III DB43/T 1968—2020 IV DB43/T 1968—2020 穗状狐尾藻生长抑制试验操作规程 1 范围 本文件规定了穗状狐尾藻（Myriophyllum Verticillatum L.）生长抑制试验的试验条件、材料的准备、 操作方法、观察记录、数据处理、质量保证与质量控制等。 本文件适用于湖南省穗状狐尾藻生长抑制试验的试验操作。 2 规范性引用文件 下列文件中的内容通过文中的规范性引用而构成本