ICS 65.020 CCS B 16 湖 43 南 省 地 方 标 准 DB43/T 2015—2021 水稻抗稻瘟病田间病圃鉴定与评价 技术规程 Technical regulation for identification and evaluation of rice blast resistance in field disease nursery 2021 - 05 - 20 实施 2021 - 02 - 20 发布 湖南省市场监督管理局 发 布 DB43/T 2015—2021 目 次 前言························································································································ Ⅲ 1 范围 ····················································································································· 1 2 规范性引用文件 ······································································································ 1 3 术语和定义 ············································································································ 1 3.1 稻瘟病 ············································································································ 1 3.2 苗瘟 ··············································································································· 1 3.3 叶瘟 ··············································································································· 1 3.4 穗瘟 ··············································································································· 1 3.5 稻瘟病圃 ········································································································· 1 3.6 抗稻瘟病鉴定 ··································································································· 1 3.7 诱发品种 ········································································································· 2 4 鉴定方法 ··············································································································· 2 4.1 病圃的选择 ······································································································ 2 4.2 苗期鉴定圃设计 ································································································ 2 4.3 成株期鉴定圃设计 ····························································································· 3 4.4 病圃的管理 ······································································································ 3 5 鉴定内容及调查时期 ································································································ 3 5.1 苗瘟鉴定 ········································································································· 3 5.2 叶瘟鉴定 ········································································································· 4 5.3 穗瘟鉴定 ········································································································· 4 6 病情调查及抗性评价分级方法 ···················································································· 5 6.1 苗叶瘟调查分级及抗性评价方法 ··········································································· 5 6.2 穗瘟调查分级及抗性评价方法 ·············································································· 5 6.3 综合指数及稻瘟病抗性综合评价 ··········································································· 5 6.4 抗性鉴定结果有效性判断 ···················································································· 5 7 档案管理 ··············································································································· 6 附录 A （规范性） 水稻稻瘟病调查及评价分级标准 ··························································· 7 附录 B （资料性） 水稻抗稻瘟病鉴定数据记载与统计表 ····················································· 9 I DB43/T 2015—2021 II DB43/T 2015—2021 前 言 本文件按照GB/T 1.1—2020《标准化工作导则 第1部分：标准化文件的结构和起草规则》的规定 起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本文件为 DB43/T 319—2006《水稻抗稻瘟病鉴定及评价方法》的修订版。本文件代替 DB43/T 319 —2006 在水稻抗稻瘟病鉴定中参考使用。本文件与 DB43/T 319—2006 相比，除编辑性修改外主要技术 内容变化如下： ——强调了苗叶瘟病级应取苗瘟与叶瘟中较重的病级； ——明确了穗瘟应包括穗颈瘟与枝梗瘟，并进一步对穗颈瘟与枝梗瘟进行了定义； ——调整了穗瘟调查时间，具体为黄熟初期（80％稻穗尖端谷粒成熟时） ； ——增加了苗期、成株期鉴定圃的田间品种布局方式各 1 种，鉴定单位可根据自身习惯选择其中一 种； ——规定了单穗病级分级调查时，先看发病部位、再看损失率，先调查穗颈瘟、再调查枝梗瘟，以 减少人为调查误差； ——将穗瘟单穗分级标准提前，穗瘟发病率及其病级可通过统计发病穗数与调查总穗数计算出，穗 瘟损失率及其病级可通过单穗病级及其对应损失率计算出； ——细化了穗瘟发病率分级标准，明确规定如为穗颈瘟的、1 个病穗记为 1 个穗瘟发病，如为枝梗 瘟的、3 个病穗记为 1 个穗瘟发病，增强了穗瘟发病率分级的合理性。 本文件的附录 A 为规范性附录，附录 B 为资料性附录。 本文件由湖南省农业农村厅提出。 本文件由湖南省农业标准化技术委员会归口。 本文件起草单位：湖南省植物保护研究所、湖南亚华种业科学研究院、湘西土家族苗族自治州农业 科学研究院、湖南省农作物良种引进示范中心。 本文件主要起草人：肖友伦、李小娟、肖放华、杨远柱、胡小淳、鲁建华、蔡义东、刘虎、张茂哲、 陆训。 本文件历次版本发布情况为： ——DB/4300 B1901-88； ——DB43/T 319—2006。 III DB43/T 2015—2021 IV DB43/T 2015—2021 水稻抗稻瘟病田间病圃鉴定与评价技术规程 1 范围 本文件规定了水稻抗稻瘟病田间病圃鉴定与评价技术规程的术语和定义、鉴定方法、鉴定内容及调 查时期、病情调查与抗性评价分级方法及档案管理等技术要求。 本文件适用于湖南省内水稻品种抗稻瘟病鉴定评价和水稻抗稻瘟病种质资源筛选。水稻品种稻瘟病 抗性比较试验与主栽品种抗性监测也可参考。 2 规范性引用文件